XBP1 antibody (N-Term)

Catalog No. ABIN501132

This anti-XBP1 antibody is a Rabbit Polyclonal antibody detecting XBP1 in WB and IF. Suitable for Human and Mouse.

Quick Overview for XBP1 antibody (N-Term) (ABIN501132)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-XBP1 Antibody

Binding Specificity: N-Term

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Affinity chromatography

Immunogen: XBP-1 antibody was raised against a 18 amino acid peptide from near the amino terminus of human XBP-1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot. Immunohistochemistry on paraffin sections: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1 / TREB5

Background: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94 (3,4).Synonyms: Tax-responsive element-binding protein 5, X-box-binding protein 1, XBP-1, XBP2

Gene ID: 7494

Pathways: ER-Nucleus Signaling, Unfolded Protein Response