ZBED1 antibody (Center)

Catalog No. ABIN501137

The Rabbit Polyclonal anti-ZBED1 antibody is suitable to detect ZBED1 in samples from Human and Mouse. It has been validated for WB and EIA.

Quick Overview for ZBED1 antibody (Center) (ABIN501137)

Target: ZBED1 (Zinc Finger, BED-Type Containing 1 (ZBED1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZBED1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ZBED1 Antibody

Binding Specificity: Center

Specificity: This antibody detects ZBED1. The antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Affinity chromatography

Immunogen: ZBED1 antibody was raised against a 15 amino acid peptide from near the center of human ZBED1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for ZBED1

Target: ZBED1 (Zinc Finger, BED-Type Containing 1 (ZBED1))

Alternative Name: ZBED1

Background: ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters, anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.Synonyms: ALTE, DREF, KIAA0785, Putative Ac-like transposable element, Zinc finger BED domain-containing protein 1, dREF homolog

Gene ID: 9189