SH3 Domain Containing Ring Finger 1 (SH3RF1) antibody

Antigen: SH3 Domain Containing Ring Finger 1 (SH3RF1)

Synonyms: POSH, RNF142, SH3MD2, FLJ21602, KIAA1494, Posh, R75531, Sh3md2, 2200003J05Rik, posh, rnf142, sh3md2, xPOSH, MGC135632, MGC189605, DKFZp469I178

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN502370

Additional Information

Alternative name: SH3RF1 / RNF142

Gene ID: SH3RF1

UniProt: Q7Z6J0

Immunogen: The immunogen for anti-SH3RF1 antibody: synthetic peptide directed towards the middle region of human SH3RF1

Sequence: LLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVG PELPPGGGHG

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-SH3RF1 antibody concentration is 1 mg/ml.

Description: SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.

Characteristics: Antigen Size: 888 amino acids

Molecular Weight: 93kDa

Application Details

Application Notes: WB Suggested Anti-SH3RF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: DU145 cell lysate.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-SH3RF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: DU145 cell lysate

Publications

Product: Lyons, Thorburn, Ryan et al.: "Regulation of the Pro-apoptotic scaffolding protein POSH by Akt." in: The Journal of biological chemistry, Vol. 282, Issue 30, pp. 21987-97, 2007 (PubMed).