SLC5A8 antibody (Middle Region)

Catalog No. ABIN502477

This Rabbit Polyclonal antibody specifically detects SLC5A8 in WB. It exhibits reactivity toward Human, Mouse, Rat and Rabbit and has been mentioned in 3+ publications.

Quick Overview for SLC5A8 antibody (Middle Region) (ABIN502477)

Target: SLC5A8 (Solute Carrier Family 5 (Iodide Transporter), Member 8 (SLC5A8))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC5A8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SLC5A8 Antibody

Binding Specificity: Middle Region

Sequence: GILVPFANSI GALVGLMAGF AISLWVGIGA QIYPPLPERT LPLHLDIQGC

Predicted Reactivity: Human: 100%, Mouse: 83%, Rabbit: 92%, Rat: 91%

Characteristics: This is a rabbit polyclonal antibody against SLC5A8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SLC5A8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 610 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SLC5A8 antibody (ABIN502477)

Elangovan, Pathania, Ramachandran, Ananth, Padia, Srinivas, Babu, Hawthorn, Schoenlein, Boettger, Smith, Prasad, Ganapathy, Thangaraju: "Molecular mechanism of SLC5A8 inactivation in breast cancer." in: Molecular and cellular biology, Vol. 33, Issue 19, pp. 3920-35, (2013) (PubMed).

Babu, Ramachandran, CoothanKandaswamy, Elangovan, Prasad, Ganapathy, Thangaraju: "Role of SLC5A8, a plasma membrane transporter and a tumor suppressor, in the antitumor activity of dichloroacetate." in: Oncogene, Vol. 30, Issue 38, pp. 4026-37, (2011) (PubMed).

Zhang, Bao, Yang, Wu, Yu, Huang, Sun, Zheng, Li: "Activin A induces SLC5A8 expression through the Smad3 signaling pathway in human colon cancer RKO cells." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 12, pp. 1964-72, (2010) (PubMed).

Target Details for SLC5A8

Target: SLC5A8 (Solute Carrier Family 5 (Iodide Transporter), Member 8 (SLC5A8))

Alternative Name: SLC5A8

Background: SLC5A8 has been shown to transport iodide by a passive mechanism (Rodriguez et al., 2002 [PubMed 12107270]) and monocarboxylates and short-chain fatty acids by a sodium-coupled mechanism (Gopal et al., 2004 [PubMed 15322102]). In kidney, SLC5A8 functions
Alias Symbols: AIT, MGC125354, SMCT, SMCT1
Protein Size: 610

Molecular Weight: 66 kDa

Gene ID: 160728

NCBI Accession: NM_145913, NP_666018

UniProt: Q8N695