NDST4 antibody (N-Term)

Catalog No. ABIN503063

The Rabbit Polyclonal anti-NDST4 antibody has been validated for WB. It is suitable to detect NDST4 in samples from Human, Mouse, Cow, Horse, Rabbit, Rat, Dog, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for NDST4 antibody (N-Term) (ABIN503063)

Target: NDST4 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 4 (NDST4))

Reactivity: Human, Mouse, Cow, Horse, Rabbit, Rat, Dog, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDST4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NDST4 Antibody

Binding Specificity: N-Term

Sequence: EDWTIFQYNH STYQPVLLTE LQTEKSLSSL SSKTLFATVI QDLGLHDGIQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 90%

Characteristics: This is a rabbit polyclonal antibody against NDST4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NDST4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 872 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NDST4

Target: NDST4 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 4 (NDST4))

Alternative Name: NDST4

Background: NDST4 is an essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. It modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. NDST4 has low deacetylase activity but high sulfotransferase activity.
Alias Symbols: NDST-4, NHSST4
Protein Size: 872

Molecular Weight: 101 kDa

Gene ID: 64579

NCBI Accession: NM_022569, NP_072091

UniProt: Q9H3R1

Pathways: Glycosaminoglycan Metabolic Process