ID1 antibody (Middle Region)

Catalog No. ABIN504674

The Rabbit Polyclonal anti-ID1 antibody has been validated for WB and IHC. It is suitable to detect ID1 in samples from Human.

Quick Overview for ID1 antibody (Middle Region) (ABIN504674)

Target: ID1 (Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ID1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ID1 Antibody

Binding Specificity: Middle Region

Sequence: IRDLQLELNS ESEVGTPGGR GLPVRAPLST LNGEISALTA EVRSRSDH

Cross-Reactivity: Human

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against ID1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ID1

Application Details

Application Notes: WB Suggested Anti-ID1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:7812500
Positive Control: Human brain.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 149 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ID1

Target: ID1 (Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1))

Alternative Name: ID1 / BHLHB24

Background: ID1 is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. It has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: ID, bHLHb24
Protein Interaction Partner: UBC, SUV39H1, PPP2CA, CAV1, TCF4, COPB1, FZR1, RASSF1, CDC20, HHV8GK18_gp81, TCF3, PSMD4, SMURF2, IKBKG, GATA4, NKX2-5, MYOD1, TCF12, ELK4, ELK1, CASK, MYF6, MYF5, MYOG, IFI16, HES1, RUNX1T1, ELSPBP1,
Protein Size: 149

Molecular Weight: 15 kDa

Gene ID: 3397

NCBI Accession: NM_181353, NP_851998

UniProt: P41134