GAPDH antibody

Catalog No. ABIN559691

Product Details anti-GAPDH Antibody

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Hamster, Saccharomyces cerevisiae, Insect, Bacteria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Immunostaining (ISt)

Specificity: Recognizes native and denatured forms of GAPDH (∼37kDa). GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster / GAPDH from other species may also be detectable

Purification: Protein A affinity chromatography from mouse ascites fluid

Immunogen: Recombinant GAPDH

Clone: GA1R

Isotype: IgG1

Application Details

Application Notes: WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test

Restrictions: For Research Use only

Handling

Concentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-GAPDH antibody (ABIN559691)

Steinbrecht, Pfeifer, Herzog, Katzenberger, Schulz, Kammerer, Küpper: "HepG2-1A2 C2 and C7: Lentivirus vector-mediated stable and functional overexpression of cytochrome P450 1A2 in human hepatoblastoma cells." in: Toxicology letters, Vol. 319, pp. 155-159, (2019) (PubMed).

Martínez-Moya, Ortega-González, González, Anzola, Ocón, Hernández-Chirlaque, López-Posadas, Suárez, Zarzuelo, Martínez-Augustin, Sánchez de Medina: "Exogenous alkaline phosphatase treatment complements endogenous enzyme protection in colonic inflammation and reduces bacterial translocation in rats." in: Pharmacological research : the official journal of the Italian Pharmacological Society, Vol. 66, Issue 2, pp. 144-53, (2012) (PubMed).

Gandhi, Takahashi, Virtanen, Syed, Davey, Mahomed: "Microarray analysis of the infrapatellar fat pad in knee osteoarthritis: relationship with joint inflammation." in: The Journal of rheumatology, Vol. 38, Issue 9, pp. 1966-72, (2011) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH (GAPDH Products)

Synonyms: G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody