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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody

Antigen

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Synonyms
G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, G ... show more
Clonality Monoclonal (GA1R)
Host
Alternatives

Mouse
Reactivity
Alternatives

Bacteria, Insect, Saccharomyces cerevisiae (S. cerevisiae), Human, Mouse (Murine), Rat (Rattus), Rabbit, Chicken, Hamster
Application
Alternatives Dot Blot (Dot), ELISA, Immunocytochemistry (ICC), Western Blotting (WB)
Catalog no. ABIN559691
Quantity 100ug  (1mg/mL)
Price 219.00 $   Plus shipping costs $35.00
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody

Additional Information
Application Details Images Alternatives Secondary Antibodies back to top

Alternative name GAPDH
Immunogen Recombinant GAPDH
Isotype IgG1  (Matching secondary antibodies)
Clone GA1R
Specificity Recognizes native and denatured forms of GAPDH (∼37kDa). GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster / GAPDH from other species may also be detectable
Synonyms G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Application Details
Images Alternatives Secondary Antibodies back to top

Application Notes WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test
Concentration 1mg/mL
Purification Protein A affinity chromatography from mouse ascites fluid
Buffer 10mM PBS, pH 7.2, 0.05% NaN3
Storage Store at -20°C for up to 2 years or more. Centrifuge after first thaw to maximize product recovery. Avoid repeated freeze-thaw cycles by aliquots. Store aliquots at 4°C for several days to weeks
Restrictions For Research Use only

Alternatives
Application Details Images Secondary Antibodies back to top

Alternatives for antigen "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)", type "Antibodies"
Hosts Rabbit (141), Mouse (123), Goat (23), Sheep (13), Chicken (2)
Reactivities Human (204), Mouse (Murine) (121), Rat (Rattus) (114), Pig (Porcine) (37), Rabbit (35), Cow (Bovine) (31), Dog (Canine) (21), Chicken (16), Goat (13), Fish (12), Escherichia Coli (E. Coli) (8), Hamster (8), Cat (Feline) (7), Monkey (7), Mammalian (6), Bird (Avian) (5), Yeast (Saccharomyces Cerevisiae) (5), Fruit Fly (Drosophila melanogaster) (3), Xenopus laevis (3), Insect (2), Sheep (Ovine) (2), Amphibian (1), Horse (Equine) (1), Zebrafish (Brachydanio rerio) (1)
Applications Western Blotting (WB) (219), Immunofluorescence (IF) (147), ELISA (88), Flow Cytometry (FACS) (80), Enzyme Immunoassay (EIA) (45), Immunoprecipitation (IP) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Immunocytochemistry (ICC) (20), Immunohistochemistry (IHC) (18), Dot Blot (Dot) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (8), Immunodiffusion (ID) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Radioimmunoassay (RIA) (6), Immunoelectron Microscopy (IEM) (3), ELISA (Detection) (2), Immunoelectron Microscopy (EM) (1)
Conjugates HRP (15), Biotin (13), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), Alexa Flour 488 (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy7 (4), RBITC (4)
Epitopes C-Term (13), N-Term (6), internal (3)