Chloride Channel 2 (CLCN2) (AA 38-387) antibody

Antigen: Chloride Channel 2 (CLCN2)

Synonyms: CLC2, ECA2, ECA3, EGI3, EGMA, EJM6, EJM8, CIC-2, EGI11, Clc2, ClC-2, nmf240, AL118368, CLCN2, CLC-2, MGC139321, Clcn2, LOC100150892

Binding Site: AA 38-387

Clonality: Monoclonal (3E1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, RNA Interference (RNAi)

Certificates: ISO 9001:2008

Catalog no.: ABIN560397

Additional Information

Alternative name: CLCN2

Gene ID: 1181, BC021578

Immunogen: CLCN2 (AAH21578.1, 38 a.a. ~ 387 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: TVSTAVIVFELTGQIAHILP VMIVVILANAVAQSLQPSLY DSIIRIKKLPYLPELGWGRH QQYRVRVEDIMVRDVPHVAL SCTFRDLRLALHRTKGRMLA LVESPESMILLGSIERSQVV ALLGAQLSPARRRQHMQERR ATQTSPLSDQEGPPTPEASV CFQVNTEDSAFPAARGETHK PLKPALKRGPSVTRNLGESP TGSAESAGIALRSLFCGSPP PEAASEKLESCEKRKLKRVR ISLASDADLEGEMSPEETHT IFSLLGVDHAYVTSIGRLIG IVTLKELRKAIEGSVTAQGV KVRPPLASFRDSATSSSDTE TTEVHALWGPHSRHGLPREG SPSDSDDKCQ

Cross-Reactivity: Human

Isotype: IgG2a kappa

Clone: 3E1

Description: Gene: chloride channel 2
Synonyms: CIC-2, CLC2, ECA3, EGI3, EGMA

Characteristics: Mouse monoclonal antibody raised against a full length recombinant CLCN2.

Application Details

Application Notes: Application specialization: Cell lysate, Recombinant protein, Transfected lysate

Buffer: 1x PBS, pH 7.2

Storage: -20 °C

Storage Comment: Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

CLCN2 monoclonal antibody (M01), clone 3E1 Western Blot analysis of CLCN2 expression in Hela NE

Western blot analysis of CLCN2 over-expressed 293 cell line, cotransfected with CLCN2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CLCN2 monoclonal antibody (M01), clone 3E1 GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1.

Lane 1: CLCN2 transfected lysate(96.8 KDa).
Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (64.24 KDa) .

Publications

Product: Alessi, Andjelkovic, Caudwell et al.: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997 (PubMed).

Datta, Dudek, Tao et al.: "Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery." in: Cell, Vol. 91, Issue 2, pp. 231-41, 1997 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, 1999 (PubMed).