The Rabbit Polyclonal anti-LDHD antibody is suitable to detect LDHD in samples from Lactobacillus leichmannii. It has been validated for WB, IP, IF, ID, RIA, EIA and DB.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Purification
Solid Phase Affinity Chromatography
Immunogen
D-Lactic dehydrogenase isolated and purified from Lactobacillus leichmannii. Freund’s complete adjuvant is used in the first step of the immunization procedure.
LDHD
Reactivity: Human
IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 680
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore by adding 0.5 mL of sterile distilled water
Concentration
2.0 mg/mL
Buffer
PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.