The Rabbit Polyclonal anti-PGK1 antibody is suitable to detect PGK1 in samples from Saccharomyces cerevisiae. It has been validated for WB, IF, IP, EIA, DB, ID and RIA.
Affinity purified antibodies of polyclonal rabbit antiserum to 3-phosphoglyceric phosphokinase from baker's yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Purification
Solid Phase Affinity Chromatography
Immunogen
3-Phosphoglyceric phosphokinase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.