Goat anti-Rabbit IgA, IgG, IgM (Fab Region), (Fc Region) Antibody (HRP)

Catalog No. ABIN568664

Quick Overview for Goat anti-Rabbit IgA, IgG, IgM (Fab Region), (Fc Region) Antibody (HRP) (ABIN568664)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Conjugate: HRP

Application: Immunofluorescence (IF), Western Blotting (WB), Immunodiffusion (ID), Dot Blot (DB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Isotype: IgG

Product Details

Binding Specificity: Fab Region, Fc Region

Reactivity: Rabbit

Host: Goat

Characteristics: Molar Ratio: Peroxidase/IgG: aprox. 1.7

Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt-Precipitation and purifaction of the IgG fraction by DEAE Chromatography

Immunogen: Purified immunoglobulin fractions isolated from pooled rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL sterile distilled water.

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -28 °C for longer.

Target Details

Target: IgA, IgG, IgM

Target Type: Antibody

Background: The reactivity of the antiserum is directed to the Fc and Fab subunits of the major Immunoglobulin isotypes. It does not react with any non-Ig protein in rabbit serum, as tested by immuno immunoelectrophoresis and double radial immunodiffusion In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in rabbit serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties.Synonyms: Rabbit IgA, Rabbit IgG, Rabbit IgM