PCBP1 antibody (Internal Region)

Catalog No. ABIN569643

The Goat Polyclonal anti-PCBP1 antibody (ABIN569643) specifically detects PCBP1 in WB and EIA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for PCBP1 antibody (Internal Region) (ABIN569643)

Target: PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PCBP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PCBP1 Antibody

Binding Specificity: AA 234-247, Internal Region

Sequence: C-ADLAKLNQVA RQQSH

Specificity: Recognizes PCBP1 (234-247).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_006187.2. Genename: PCBP1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PCBP1

Target: PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))

Alternative Name: PCBP1

Background: The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.Synonyms: Alpha-CP1, HNRPE1, HNRPX, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3, Poly(rC)-binding protein 1, SUB2.3, hnRNP E1

Gene ID: 5093

NCBI Accession: NP_006187

UniProt: Q15365