WFDC2 antibody (Internal Region)

Catalog No. ABIN569663

The Goat Polyclonal anti-WFDC2 antibody is suitable to detect WFDC2 in samples from Human and Dog. It has been validated for WB and EIA.

Quick Overview for WFDC2 antibody (Internal Region) (ABIN569663)

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This WFDC2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-WFDC2 Antibody

Binding Specificity: AA 29-43, Internal Region

Sequence: GAEKTGVCPE LQADQ

Specificity: Recognizes WFDC2 / WAP5 (29-43).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_006094.3. Genename: WFDC2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for WFDC2

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Alternative Name: WFDC2 / WAP5

Background: The whey acidic protein (WAP) domain is a conserved motif, containing eight cysteines found in a characteristic 4-disulphide core arrangement, that is present in a number of otherwise unrelated proteins. One of these proteins, which contains two WAP domains, is HE4 (also known as WFDC2), originally described as an epididymis specific protein but more recently suggested to be a putative serum tumour marker for ovarian cancer and a presumptive role in natural immunity. The HE4 protein expression is not only confined to epidydimis but is expressed in a number of normal human tissues out side the reproductive system, including regions of the respiratory tract and nasopharynx and in a subset of lung tumor cell lines. HE4 gene expression was highest in normal human trachea and salivary gland, and to a lesser extent, lung, prostate, pituitary gland, thyroid, and kidney. Highest level of expression of ESPE4 was observed in adenocarcinomas of the lung, and occasional breast, transitional cell and pancreatic carcinomas. The WFDC2 gene under goes extensive splicing in malignant tissues that give rise to five WAP domain containing iso forms. WFDC2 is expressed in some epithelial cells of the upper airways as well as in mucous cells and ducts of submucosal glands. No staining was seen in peripheral lung. Intense staining is found in major salivary glands and in minor glands of the nose, sinuses, posterior tongue and tonsil. Studies with the related protein Secretory Leukocyte Protease Inhibitor (SLPI) show that although both proteins are expressed in similar tissues, the precise cellular localization differs. Significant increases in expression and localization of WFDC2 are seen in patients with Cystic Fibrosis. ESPE2 protien is a 124 amino acids (15 kDa) protein. ESPE4 protein has sequence homology to extracellular proteinase inhibitor and is localized on human chromosome 20. The ESPE4 protein is a secreted glycoprotein (Asn-44) that is over expressed in serous and endometrioid ovarian carcinomas. There are at least 5 named variants (HE4-V1 to HE4-V5) expressed as a result of alternate splicing and they serve as endopeptidase inhibitors.Synonyms: Epididymal secretory protein E4, HE4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WAP four-disulfide core domain protein 2

Gene ID: 10406

NCBI Accession: NP_006094

UniProt: Q14508