ITIH4 antibody (Internal Region)

Catalog No. ABIN570706

This Goat Polyclonal antibody specifically detects ITIH4 in IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for ITIH4 antibody (Internal Region) (ABIN570706)

Target: ITIH4 (Inter alpha Globulin Inhibitor H4 (ITIH4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ITIH4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-ITIH4 Antibody

Binding Specificity: Internal Region

Purpose: ITIH4

Sequence: ERLCVDPRHR QGPVN

Specificity: The immunizing peptide represents a region of the 35 kDa chain. This chain is glycosylated giving rise to an apparent higher molecular weight.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ERLCVDPRHRQGPVN, from the internal region of the protein sequence according to NP_002209.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 4-6 μg/mL.

Western Blot: Preliminary experiments gave an approx. 150 kDa band in Human Liver and Lung lysates after 0.03 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated s

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ITIH4

Target: ITIH4 (Inter alpha Globulin Inhibitor H4 (ITIH4))

Alternative Name: ITIH4

Background: ITIH4, inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein), DKFZp686G21125, H4P, IHRP, ITIHL1, PK120, inter-alpha (globulin) inhibitor H4, plasma kallikrein-sensitive glycoprotein

Gene ID: 3700

NCBI Accession: NP_002209

Pathways: Glycosaminoglycan Metabolic Process