TRPC4 antibody (Internal Region)

Catalog No. ABIN570934

This Goat Polyclonal antibody specifically detects TRPC4 in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for TRPC4 antibody (Internal Region) (ABIN570934)

Target: TRPC4 (Transient Receptor Potential Cation Channel, Subfamily C, Member 4 (TRPC4))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TRPC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-TRPC4 Antibody

Binding Specificity: Internal Region

Purpose: TRPC4

Sequence: KHAKEEDSSI DYD

Specificity: This antibody is expected to recognise all six reported isoforms (NP_057263.1, NP_003297.1, NP_001129427.1, NP_001129429.1, NP_001129428.1, NP_001129430.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KHAKEEDSSIDYD, from the internal region (near C Terminus) of the protein sequence according to NP_057263.1, NP_003297.1, NP_001129427.1, NP_001129429.1, NP_001129428.1, NP_001129430.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta and Kidney. Recommended concentration: 4.75 μg/mL.

Western Blot: Approx. 110 kDa band observed in Rat Brain lysates (calculated MW of 112 kDa according to Human NP_057263.1 and to Rat NP_536321.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. This antibody has been successfully us

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRPC4

Target: TRPC4 (Transient Receptor Potential Cation Channel, Subfamily C, Member 4 (TRPC4))

Alternative Name: TRPC4

Background: TRPC4, transient receptor potential cation channel, subfamily C, member 4, HTRP4, MGC119570, MGC119571, MGC119572, MGC119573, TRP4, transient receptor potential 4

Gene ID: 7223, 22066, 84494

NCBI Accession: NP_057263, NP_003297, NP_001129427, NP_001129429, NP_001129428, NP_001129430