PSMB8 antibody (C-Term)

Catalog No. ABIN571001

The Goat Polyclonal anti-PSMB8 antibody is suitable to detect PSMB8 in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for PSMB8 antibody (C-Term) (ABIN571001)

Target: PSMB8 (Proteasome (Prosome, Macropain) Subunit, beta Type, 8 (Large Multifunctional Peptidase 7) (PSMB8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PSMB8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PSMB8 Antibody

Binding Specificity: C-Term

Purpose: LMP7

Sequence: DVSDLLHQYR EANQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_004150.1, NP_683720.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DVSDLLHQYREANQ, from the C Terminus of the protein sequence according to NP_004150.1, NP_683720.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 22 kDa band observed in Human Liver, Lung and Lymph Node lysates (calculated MW of 29.8 kDa according to precursor NP_004150.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sch

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PSMB8

Target: PSMB8 (Proteasome (Prosome, Macropain) Subunit, beta Type, 8 (Large Multifunctional Peptidase 7) (PSMB8))

Alternative Name: PSMB8

Background: PSMB8, proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7), D6S216, D6S216E, LMP7, MGC1491, PSMB5i, RING10, beta5i, OTTHUMP00000029420, OTTHUMP00000029421, OTTHUMP00000062981, low molecular weight protein 7, macropain

Gene ID: 5696, 16913, 24968

NCBI Accession: NP_004150, NP_683720

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA