OCT4 antibody (Internal Region)

Catalog No. ABIN571163

The Goat Polyclonal anti-OCT4 antibody is suitable to detect OCT4 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for OCT4 antibody (Internal Region) (ABIN571163)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-OCT4 Antibody

Binding Specificity: Internal Region

Purpose: OCT4 / POU5F1 (mouse)

Sequence: DRPNAVKLEK VEP

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-DRPNAVKLEKVEP

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38-40 kDa band observed in lysates of cell line NIH3T3 and approx. 45 kDa in lysates of cell line HepG2 (calculated MW of 38.6 kDa according to Human NP_002692.2 and 38.2 kDa according to Mouse NP_038661.2). This molecular weight is rout

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: POU5F1

Background: Pou5f1, POU domain, class 5, transcription factor 1, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4

Gene ID: 18999

NCBI Accession: NP_038661

Pathways: Stem Cell Maintenance