E. Coli Elongation Factor TU antibody

Catalog No. ABIN594768

The Mouse Monoclonal anti-E. Coli Elongation Factor TU antibody (Clone 9E2) (ABIN594768) specifically detects E. Coli Elongation Factor TU in IF, ELISA and WB. The antibody is reactive with Bacillus, Deinococcus, E. coli, Pseudomonas, Pseudomonas cepacia (Burkholderia cepacia) and Streptococcus samples.

Quick Overview for E. Coli Elongation Factor TU antibody (ABIN594768)

Target: E. Coli Elongation Factor TU

Reactivity: Bacillus, Deinococcus, E. coli, Pseudomonas, Pseudomonas cepacia (Burkholderia cepacia), Streptococcus

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), ELISA, Western Blotting (WB)

Clone: 9E2

Product Details

Specificity: Recognizes Escherichia coli Elongation Factor TU at the very end of the N-terminus of the EF-Tu molecule (SKEKFE). Species cross-reactivity: Bacteroides fragilis, Streptococcus oralis, Bacillus subtilis, Pseudomonas aeruginosa, Burkholderia cepacia, Deinococcus sp., and weakly cross-reacts with Mycobacterium tuberculosis.

Purification: Protein G purified

Immunogen: E. coli K-12 C600 Rif (pKT146) bacteria.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IF, WB (1:50)

Usage: Suitable for use in ELISA, Immunofluorescence and Western Blot. Western Blot: 1:50 Bacterial EF-Tu reveals a molecular mass of 43-47kD, whereas archaeal EF-Tu reveals as either 80 or 60kD (ref 1).

Comment:

Target Species of Antibody: E. coli

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: E. Coli Elongation Factor TU