DC-SIGN/CD209 antibody
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- Target See all DC-SIGN/CD209 (CD209) Antibodies
- DC-SIGN/CD209 (CD209) (CD209)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This DC-SIGN/CD209 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA
- Specificity
- Recognizes human DC-SIGN on transfected NIH/3T3 cells and on monocyte derived dendritic cells at 44kD. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
- Purification
- Protein G purified
- Immunogen
- NIH/3T3 transfected with recombinant, human DC-SIGN.
- Isotype
- IgG2b
- Top Product
- Discover our top product CD209 Primary Antibody
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- Application Notes
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Approved: ELISA, Flo, IHC (25 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Adhesion Blockade and Flow Cytometry. Flow Cytometry (Immunophenotyping): 10 μL of 25-50 μg/mL labels up to 10^6 cells. Adhesion Blockade: 5 μg/mL (5x10^6 cells/well). Immunohistochemistry: 25 μg/mL detects human DC-SIGN in fixed cells and tissue sections. Western Blot: 1-2 μg/mL The detection limit is a ~5 ng/well under non-reducing and reducing conditions using a colorimetric detection system,. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile 40-50 % glycerol, PBS.
- Concentration
- Lot specific
- Buffer
- Lyophilized from in PBS, pH 7.2, 5 % trehalose
- Storage
- -20 °C
- Storage Comment
- Lyophilized powder may be stored at -20°C for short-term only. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
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- Target
- DC-SIGN/CD209 (CD209) (CD209)
- Alternative Name
- CD209 / DC-SIGN (CD209 Products)
- Background
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Name/Gene ID: CD209
Synonyms: CD209, CD209 antigen, CD209 Molecule, CDSIGN, CLEC4L, DC-SIGN, HIV gpl20-binding protein, DC-SIGN1 - Gene ID
- 30835
- UniProt
- Q9NNX6
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