SEMA3F antibody (AA 227-276)

Catalog No. ABIN6746110

The Rabbit Polyclonal anti-SEMA3F antibody is suitable to detect SEMA3F in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Hamster and Pig. It has been validated for WB.

Quick Overview for SEMA3F antibody (AA 227-276) (ABIN6746110)

Target: SEMA3F (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Hamster, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA3F antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SEMA3F Antibody

Binding Specificity: AA 227-276

Specificity: Mouse SEMA3F

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Horse (92%) Chicken, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa227-276 of mouse Sema3f (O88632-2, NP_035479). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Guinea pig, Seabass (100%), Marmoset, Horse, Opossum, Platypus (92%), Lizard, Xenopus (91%), Zebrafish (90%), Turkey, Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SEMA3F

Target: SEMA3F (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))

Alternative Name: SEMA3F

Background: Name/Gene ID: SEMA3F

Synonyms: SEMA3F, Semaphorin III/F, Semaphorin IV, Semaphorin-3F, Sema III/F, Sema IV, SEMA-IV, SEMA4, SEMAK

Gene ID: 6405

NCBI Accession: NP_035479

UniProt: Q13275

Pathways: Regulation of Cell Size