PCNP antibody (C-Term)

Catalog No. ABIN6746506

Cited in 3+ publications. The Rabbit Polyclonal anti-PCNP antibody (ABIN6746506) specifically detects PCNP in WB. The antibody is reactive with Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Pig, Chicken and Hamster samples.

Quick Overview for PCNP antibody (C-Term) (ABIN6746506)

Target: PCNP (PEST Proteolytic Signal Containing Nuclear Protein (PCNP))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Pig, Chicken, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PCNP Antibody

Binding Specificity: C-Term

Specificity: Mouse PCNP

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Chicken (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of mouse Pcnp (Q6P8I4, NP_001019793). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Monkey, Xenopus (92%), Salmon, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PCNP antibody (ABIN6746506)

Bolton, Anthony, Perry: "Loss of the tight junction proteins occludin and zonula occludens-1 from cerebral vascular endothelium during neutrophil-induced blood-brain barrier breakdown in vivo." in: Neuroscience, Vol. 86, Issue 4, pp. 1245-57, (1998) (PubMed).

Fanning, Jameson, Jesaitis, Anderson: "The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29745-53, (1998) (PubMed).

Ando-Akatsuka, Saitou, Hirase, Kishi, Sakakibara, Itoh, Yonemura, Furuse, Tsukita: "Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 43-7, (1996) (PubMed).

Target Details for PCNP

Target: PCNP (PEST Proteolytic Signal Containing Nuclear Protein (PCNP))

Alternative Name: PCNP

Background: Name/Gene ID: PCNP

Synonyms: PCNP

Gene ID: 57092

NCBI Accession: NP_001019793

UniProt: Q8WW12