ART1 antibody (C-Term)

Catalog No. ABIN6746775

This Rabbit Polyclonal antibody specifically detects ART1 in WB. It exhibits reactivity toward Human, Mouse, Cow, Dog, Guinea Pig and Rat and has been mentioned in 4+ publications.

Quick Overview for ART1 antibody (C-Term) (ABIN6746775)

Target: ART1 (ADP-Ribosyltransferase 1 (ART1))

Reactivity: Human, Mouse, Cow, Dog, Guinea Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ART1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ART1 Antibody

Binding Specificity: C-Term

Specificity: Human ART1

Predicted Reactivity: Percent identity by BLAST analysis: Guinea pig (100%) Horse (92%) Bovine (85%) Mouse, Rat, Dog (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ART1 (P52961, NP_004305). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Guinea pig (100%), Marmoset, Elephant, Horse (92%), Bovine (85%), Mouse, Rat, Dog, Pig (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-ART1 antibody (ABIN6746775)

Ordentlich, Downes, Xie, Genin, Spinner, Evans: "Unique forms of human and mouse nuclear receptor corepressor SMRT." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 2639-44, (1999) (PubMed).

Wagner, Norris, Knotts, Weigel, McDonnell: "The nuclear corepressors NCoR and SMRT are key regulators of both ligand- and 8-bromo-cyclic AMP-dependent transcriptional activity of the human progesterone receptor." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1369-78, (1998) (PubMed).

Nagy, Kao, Chakravarti, Lin, Hassig, Ayer, Schreiber, Evans: "Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase." in: Cell, Vol. 89, Issue 3, pp. 373-80, (1997) (PubMed).

Chen, Evans: "A transcriptional co-repressor that interacts with nuclear hormone receptors." in: Nature, Vol. 377, Issue 6548, pp. 454-7, (1995) (PubMed).

Target Details for ART1

Target: ART1 (ADP-Ribosyltransferase 1 (ART1))

Alternative Name: ART1 / ART2

Background: Name/Gene ID: ART1

Synonyms: ART1, ADP-ribosyltransferase 2, ART2, ARTC1, CD296, CD296 antigen, ADP-ribosyltransferase 1, Mono(ADP-ribosyl)transferase 1, RT6

Gene ID: 417

NCBI Accession: NP_004305

UniProt: P52961