eIF4EBP2 antibody (AA 99-120)

Catalog No. ABIN615304

This Rabbit Polyclonal antibody specifically detects eIF4EBP2 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for eIF4EBP2 antibody (AA 99-120) (ABIN615304)

Target: eIF4EBP2 (EIF4EBP2) (Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eIF4EBP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-eIF4EBP2 Antibody

Binding Specificity: AA 99-120

Specificity: Detects 17 and 20 kDa proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Porcine, Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: EIF4EBP2 antibody was raised against human PHAS-II synthetic peptide (residues 99-120) was synthesized and the peptide coupled to KLH. .Remarks: This sequence is 96% homologous to mouse PHAS-11 over these residues

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for eIF4EBP2

Target: eIF4EBP2 (EIF4EBP2) (Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2))

Alternative Name: 4E-BP2

Background: PHAS-II, also known as eIF4E-binding protein II (eIF4E-BPII), is a member of a family of proteins which regulate initiation. PHAS-I and -II were found to have overlapping but different patterns of expression in tissues. PHAS-II shows the highest expression in liver and kidney where very little PHAS-I is found. The PHAS proteins regulate translation initiation by binding to the inhibitory protein eIF- 4E and blocking translation by preventing access of eIF-4G to the 5' cap of the mRNA. Both PHAS proteins are phosphorylated in response to insulin or growth factors such as EGF, PDGF and IGF-1. Phosphorylation in the appropriate site(s) promotes dissociation of PHAS/eIF-4E complexes which allows eIF-4E to bind eIF-4G(p220), thereby increasing the amount of eIF-4F complex and the rate of translation initiation. Regulation of the two protein differ because PHAS-II, unlike PHAS-I is readily phosphorylated by PKA in vitro. However increasing cAMP in cells promotes dephosphorylation of both PHAS-I and PHAS-II. Pharmacological and genetic evidence indicates that the mTOR/p70S6K pathway is involved in the control of PHAS-I and -II suggesting that these proteins may be mediators of the effects of this pathway on protein synthesis and cell proliferation.Synonyms: EIF4EBP2, Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2

Gene ID: 1979

NCBI Accession: NP_004087

UniProt: Q13542