Sheep anti-Mouse IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Catalog No. ABIN616117

Quick Overview for Sheep anti-Mouse IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed (ABIN616117)

Target: IgG

Clonality: Polyclonal

Conjugate: FITC

Application: Immunoassay (IA)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Sheep

Characteristics: Molar Ratio: 2.2 moles FITC per mole of Sheep IgG.

Purification: Preadsorption: Solid phase absorption

Immunogen: Mouse IgG whole molecule.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Absorption / Emission: 495 nm / 528 nm

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

References

Carvalho, Walter, Baermann-Stapel, Weller, Panne, Schenk, Schneider: "Non-invasive monitoring of immunization progress in mice via IgG from feces." in: In vivo (Athens, Greece), Vol. 26, Issue 1, pp. 63-9, (2012) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Synonyms: Mouse Immunoglobulin G