Sheep anti-Mouse IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed

Catalog No. ABIN616120

Quick Overview for Sheep anti-Mouse IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed (ABIN616120)

Target: IgG

Clonality: Polyclonal

Conjugate: TRITC

Application: Immunoassay (IA)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Sheep

Characteristics: Molar Ratio: 3.2 moles TRITC per mole of Goat IgG.

Purification: Preadsorption: Solid phase absorption

Immunogen: Mouse IgG whole molecule.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Absorption / Emission: 550 nm / 570 nm

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

References

Carvalho, Walter, Baermann-Stapel, Weller, Panne, Schenk, Schneider: "Non-invasive monitoring of immunization progress in mice via IgG from feces." in: In vivo (Athens, Greece), Vol. 26, Issue 1, pp. 63-9, (2012) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Synonyms: Mouse Immunoglobulin G