Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN619407

Quick Overview for Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN619407)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Rabbit

Purpose: Rabbit anti-Human IgG (H&L), ALP conjugated

Purification: Immunogen affinity purified

Immunogen: Purified human IgG (H&L) CAA10835

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunogen affinity purified rabbit IgG.

Handling Advice: Prepare working dilution prior to use and discard after use. Do not store working dilution,Be sure to mix well but without foaming

Storage: 4 °C,-20 °C

Storage Comment: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Expiry Date: 48 months

Target Details

Target: IgG

Target Type: Antibody

Background:

Background: Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to ALP, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).

Additional information: Purity of this preparation is > 95 % based on SDS-PAGE. Antibody concentration is 1.50 mg/mL (E 1 % at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins