Histone 3 antibody

Catalog No. ABIN619545

Cited in 2+ publications. The Rabbit Polyclonal anti-Histone 3 antibody (ABIN619545) specifically detects Histone 3 in WB, IF, ICC and ChIP-qPCR. The antibody is reactive with Human, Arabidopsis thaliana, Oryza sativa, Tomato, Zea mays, Chlamydomonas reinhardtii, Phaeodactylum tricornutum, Phalaenopsis equestris, Nicotiana benthamiana, Botrytis cinerea (Grey Mold), Cabbage, Camellia sinensis, Chickpea, Barley, Chlamydomonas acidophila, Cucumis sativus, Cucurbita pepo, Physcomytrium Patens, Potato, Red pepper, Salicornia europaea, Solanum sogarandinum, Stevia rebaudiana and Vicia faba samples.

Quick Overview for Histone 3 antibody (ABIN619545)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Arabidopsis thaliana, Oryza sativa, Tomato, Zea mays, Chlamydomonas reinhardtii, Phaeodactylum tricornutum, Phalaenopsis equestris, Nicotiana benthamiana, Botrytis cinerea (Grey Mold), Cabbage, Camellia sinensis, Chickpea, Barley, Chlamydomonas acidophila, Cucumis sativus, Cucurbita pepo, Physcomytrium Patens, Potato, Red pepper, Salicornia europaea, Solanum sogarandinum, Stevia rebaudiana, Vicia faba

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ChIP quantitative PCR (ChIP-qPCR)

Product Details anti-Histone 3 Antibody

Purpose: Anti-H3 | Histone H3 (rabbit antibody) (nuclear marker)

Cross-Reactivity (Details): Arabidopsis thaliana, Botrytis cinerea, Brassica oleracea, Camellia sinensis, Capsicum annuum, Cicer arietinum L., Chlamydomonas acidophila, Chlamydomonas reinhardtii, Cucumis sativus L cv Suyo, Cucurbita pepo L. var. cylindrica, Hordeum vulgare, human, Nicotiana benthamiana, Oryza sativa, Phalaenopsis equestris, Phaeodactylum tricornutum, Physcomitrium patens, Salicornia europaea, Stevia rebaudiana, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Brachypodium distachyon,Brassica napus,Emiliania huxleyi,Fagopyrum tataricum Gaertn.,Glycine max,Nannochloropsis gaditana,Nicotiana tabacum,Malus domestica,Medicago sativa,Oryza sativa,Ostreococcus sp.,Pinus pinaster,Pisum sativum,Porphyridium purpureum,Saccharum sp.,Sorghum bicolor,Triticum aestivum,Vitis vinifera,Volvox sp.

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide derived from known H3 sequences

Application Details

Application Notes: 1 : 100-1 : 500 (ICC), 2 μL of antibody/500 μL solution (ChIp-qPCR), 1: 500 (IF), 1 : 5000 (WB)

Comment:

Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-Histone 3 antibody (ABIN619545)

Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

van der Linde, Hemetsberger, Kastner, Kaschani, van der Hoorn, Kumlehn, Doehlemann: "A maize cystatin suppresses host immunity by inhibiting apoplastic cysteine proteases." in: The Plant cell, Vol. 24, Issue 3, pp. 1285-300, (2012) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: H3

Background:

Background: Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Additional information: Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii

Molecular Weight: 15 | 17 kDa

Gene ID: 830164

UniProt: P59169, P59226, Q9FXI7