Chicken anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN619633

Quick Overview for Chicken anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN619633)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Isotype: IgY

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Chicken

Purpose: Chicken anti-Rabbit IgG (H&L), ALP conjugated

Cross-Reactivity (Details): Rabbit IgG (H&L)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Rabbit IgG (H,L)

Purification: Immunogen affinity purified

Immunogen: Purified Rabbit IgG

Application Details

Application Notes: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Comment:

This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunogen affinity purified chicken IgY.

Handling Advice: Prepare working dilution prior to use and discard after use. Do not store working dilution,Be sure to mix well but without foaming

Storage: 4 °C,-20 °C

Storage Comment: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Expiry Date: 48 months

Target Details

Target: IgG

Target Type: Antibody

Background:

Background: Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.

Additional information: ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative