SH3RF1 antibody (Middle Region)

Catalog No. ABIN631195

This Rabbit Polyclonal antibody specifically detects SH3RF1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SH3RF1 antibody (Middle Region) (ABIN631195)

Target: SH3RF1 (SH3 Domain Containing Ring Finger 1 (SH3RF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3RF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SH3RF1 Antibody

Binding Specificity: Middle Region

Specificity: SH3 RF1 antibody was raised against the middle region of SH3 F1

Purification: Affinity purified

Immunogen: SH3 RF1 antibody was raised using the middle region of SH3 F1 corresponding to a region with amino acids LLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHG

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

SH3RF1 Blocking Peptide, (ABIN940541), is also available for use as a blocking control in assays to test for specificity of this SH3RF1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of SH0 F1 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for SH3RF1

Target: SH3RF1 (SH3 Domain Containing Ring Finger 1 (SH3RF1))

Alternative Name: SH3RF1

Background: SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.

Molecular Weight: 93 kDa (MW of target protein)