OCT4 antibody

Catalog No. ABIN636369

The Rabbit Polyclonal anti-OCT4 antibody (ABIN636369) specifically detects OCT4 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for OCT4 antibody (ABIN636369)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-OCT4 Antibody

Cross-Reactivity (Details): This antibodys immunogen sequence shares 84 % sequence identity with the Mouse homolog, and 94 % sequence identity with the rat homolog.

Purification: Affinity chromatography purified

Immunogen: Oct 3/4 antibody was raised in rabbit using an internal sequence of the human Oct 3/4 protein as the immunogen.

Isotype: IgG

Application Details

Application Notes: IHC: 1:100-1:250, WB: 1:500-1:1,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Affinity purified and supplied in PBS, with 0.02 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct 3/4

Background: Oct3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct3/4 induces loss of pluripotency and dedifferentiation to trophectoderm.

Pathways: Stem Cell Maintenance