CD3 (CD3E) antibody

Antigen: CD3 (CD3E)

Synonyms: T3E, TCRE, FLJ18683

Clonality: Monoclonal (MEM-57)

Host: Please enquire

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Catalog no.: ABIN638428

Additional Information

Alternative name: CD3

Gene ID: 916

Immunogen: Human thymocytes, T lymphocytes.

Endotoxin Level: low endotoxin

Isotype: IgG2a

Clone: MEM-57

Specificity: The antibody MEM-57 reacts with gamma-epsilon, delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes, mature thymocytes.HLDA IV., WS Code T 96

Application Details

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 micrometer filter sterilized. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.

Preservative (Components): Azide free

Storage: Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot, store at -20°C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Publications

Product: Soucek, Chudomel, Hrubá et al.: "Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3." in: Neoplasma, Vol. 38, Issue 1, pp. 33-41, 1991 (PubMed).

Horejsí, Angelisová, Bazil et al.: "Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74)." in: Folia biologica, Vol. 34, Issue 1, pp. 23-34, 1988 (PubMed).

Soucek, Hilgert, Budová et al.: "Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2." in: Neoplasma, Vol. 41, Issue 2, pp. 75-81, 1994 (PubMed).

Hilgert, Franĕk, Stefanová et al.: "Therapeutic in vivo use of the A1-CD3 monoclonal antibody." in: Transplantation, Vol. 55, Issue 2, pp. 435-8, 1993 (PubMed).

Dave, Cao, Browne et al.: "CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage." in: The EMBO journal, Vol. 16, Issue 6, pp. 1360-70, 1997 (PubMed).

Panyi, Bagdány, Bodnár et al.: "Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2592-7, 2003 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).