M13 Bacteriophage antibody

Antigen: M13 Bacteriophage

Host: Mouse

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Catalog no.: ABIN639403

Additional Information

Characteristics: Mouse Anti-M13 Bacteriophage

Reconstitution: Supplied as 0.25 mg of purified liquid antibody in PBS, containing 0.09% Sodium Azide. Its final concentration is 0.5 mg/ml. Please avoid freeze-thaw cycles.

Isotype: IgG2a  (Matching secondary antibodies)

Description: M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.

Specificity: DS-MB-01818 recognises the M13 bacteriophage, widely used in the preparation of 'phage-display' immunoglobulin libraries. This antibody may be used in a range of applications to detect binding of ScFv antibodies developed in the M13 system.

Application Details

Purification: Purified

Storage: Store at +4°C or at -20°C if preferred. This product should be stored undiluted.

Restrictions: For Research Use only