F-Actin antibody
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Host
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Reactivity
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Application
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 Alternatives ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
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| Catalog no. |
ABIN640417 |
| Quantity |
0.5 mg (Variants) |
| Price |
408.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Characteristics
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Mouse Anti-Human F-Actin
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Reconstitution
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Antibody is supplied as 500 mg purified IgM in phosphate-buffered saline pH 7.4, with 0.09% sodium azide Its final concentration is 1.0 mg/mL.
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Isotype
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IgM (Matching secondary antibodies)
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Description
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Actin is a globular, 42-47 kDa, structural protein found in eukaryotic cells. Actin has two main functions in cells. In all cells it forms the thinnest part of the cytoskeleton, which allows motility, while in muscle cells it also associates with myosin proteins to form the contractile apparatus. Individual subunits of actin are known as globular actin (G-actin). G-actin subunits assemble into long filamentous polymers called F-actin. Because it interacts with a large number of other proteins, actin is one of the most highly conserved throughout evolution. Mammals have at least six actin isoforms coded by separate genes, which are divided into three classes (alpha, beta and gamma) according to their isoelectric point. Alpha actins are generally found in muscle, whereas beta and gamma isoforms are prominent in non-muscle cells. Although there are small differences in sequence and properties between the isoforms, all actins assemble into microfilaments and are essentially identical in the majority of tests performed in vitro.
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Specificity
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This antibody recognizes human filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types. Cross-reacts with rabbit F-actin
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Purification
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Purified
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Storage
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If not intended for use within a month, store at -20 ° C,. Otherwise, keep it at 4 ° C and minimize freezing and thawing when use. Should this product contain a precipitate we recommend microcentrifugation before use. Stable for 18 months. Please avoid freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "F-Actin", type "Antibodies"
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Hosts
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Rabbit (20), Mouse (1)
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Reactivities
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Human (21), Mouse (Murine) (20), Rat (Rattus) (20), Cow (Bovine) (16), Pig (Porcine) (15), Sheep (Ovine) (15), Chicken (1), Dog (Canine) (1)
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Applications
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Flow Cytometry (FACS) (19), Immunofluorescence (IF) (18), ELISA (3), Western Blotting (WB) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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