Apelin (APLN) antibody
| Antigen |
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| Synonyms |
Apel, 6030430G11Rik, XNPEP2, APLN, Xapelin, xnpep2, preproapelin |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (12), Enzyme Immunoassay (EIA) (5), ELISA (4), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN641179 |
| Quantity |
200µg |
| Price |
300.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Characteristics
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Rabbit Anti-Human Apelin & Rat
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Alternative name
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Apelin
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Swiss-Prot
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NM_017413
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Reconstitution
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Supplied as purified and lyophilized antibody in sterile PBS (pH 7.5), containing 0.1% BSA. It final concentration is indicated in shipping vial. Please avoid freeze-thaw cycles.
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Apelin, an endogenous ligand for the G-protein-coupled APJ receptor, has been recently extensively studied in obesity research. It is not only expressed in adipocyte tissue, but also widely expressed in various other organs such as the heart, lung, kidney, gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma. Apelin is derived from a 77-amino-acid prepropeptide that is cleaved into a 55-amino-acid fragment and then into shorter forms. The physiologically active form is thought to be apelin 36, although the pyroglutamylated form of Apelin 13, which is also produced endogenously, is more potent. Studies have shown the association between Apelin and obesity. Apelin has higher circulating levels in obesity. Insulin exerts a positive action on adipocyte apelin production. Apelin also regulates fluid homeostasis, playing an important role in the hypothalamic regulation of food and water intake, and pituitary hormone release.
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Specificity
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The rabbit anti-human Apelin antibody detected specifically human recombinant protein and cellular protein from cell lysates at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
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Storage
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Always prepare single-use aliquots planning use while receiving package. A small amount of antibody can be kept at 4 ° C for daily use. For a long storage, recommend to keep them at -20 ° C after adding an equal volume of glycerol in PBS. It will not lose activity over a year. Minimize freezing and thawing when use.
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Research Area
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Cardiovascular, Contractility
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Restrictions
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For Research Use only
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Alternatives for antigen "Apelin (APLN)", type "Antibodies"
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Hosts
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Rabbit (26), Mouse (5)
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Reactivities
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Human (30), Mouse (Murine) (22), Rat (Rattus) (22), Cow (Bovine) (4), Dog (Canine) (2), Pig (Porcine) (2), Chicken (1), Horse (Equine) (1), Rabbit (1)
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Applications
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Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (12), Enzyme Immunoassay (EIA) (5), ELISA (4), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Alternatives 
