GAPDH antibody (C-Term)

Catalog No. ABIN644070

The Rabbit Polyclonal anti-GAPDH antibody (ABIN644070) specifically detects GAPDH in WB. The antibody is reactive with Human samples.

Quick Overview for GAPDH antibody (C-Term) (ABIN644070)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GAPDH Antibody

Binding Specificity: C-Term

Purpose: Rabbit Anti-Human GAPDH (C-term)

Specificity: DS-PB-00847 recognizes an epitope within the C-terminal region (CT) of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme which plays a key role in energy production, and has also been implicated in numerous cellular processes. GAPDH is a homotetramer molecule consisting of four 36kDa subunits, constitutively expressed in most cells and tissues, and is responsible for the generation of energy during carbohydrate metabolism, catalyzing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate. The role of GAPDH as a multifunctional protein has emerged from many studies, confirming its involvement in critical nuclear pathways, apoptosis, membrane transport and fusion, DNA replication and repair and phosphotransferase activity. Furthermore, the ability of GAPDH to bind with high affinity to beta-amyloid precursor protein (Alzheimer's disease) and selectively with CAG mutated proteins, including huntingtin (Huntingdon's disease) and the androgen receptor (spinobulbar muscular atrophy), has focused many studies towards the role of GAPDH in the pathogenesis of neurodegenerative diseases (1). Western BlotDS-PB-00847 detects a band of approximately 37kDa in HeLa cell lysates.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Purified

Immunogen: Antisera to human GAPDH (CT) were raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG prepared by affinity chromatography.

Isotype: IgG

Application Details

Application Notes: Western Blotting

Restrictions: For Research Use only

Handling

Concentration: IgG concentration 1.0 mg/ml

Buffer: Phosphate buffered saline

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH