BCL2-Related Protein A1 (BCL2A1) (Center) antibody

Antigen: BCL2-Related Protein A1 (BCL2A1)

Synonyms: GRS, BFL1, ACC-1, ACC-2, HBPA1, BCL2L5, Bcl2a1, BCL2A1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650621

Additional Information

Alternative name: BCL2A1

Gene ID: 597

UniProt: Q16548

Immunogen: This BCL2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63~92 amino acids from the Center region of human BCL2A1.

Isotype: Ig

Description: Other names: Human Bcl2-related Protein A1, BFL1, Hematopoietic-specific early response protein, GRS protein

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BCL2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63~92 amino acids from the Center region of human BCL2A1.

Molecular Weight: 20132 DA

Comments:

Background: Bcl-2 related Protein A1 is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and has been shown to be up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cancer, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of BCL2A1 Antibody (Center) (ABIN650621) in T47D cell line lysates (35ug/lane). BCL2A1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCL2A1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

BCL2A1 Antibody (Center) (ABIN650621) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Akari, Bour, Kao et al.: "The human immunodeficiency virus type 1 accessory protein Vpu induces apoptosis by suppressing the nuclear factor kappaB-dependent expression of antiapoptotic factors." in: The Journal of experimental medicine, Vol. 194, Issue 9, pp. 1299-311, 2001 (PubMed).

Akatsuka, Nishida, Kondo et al.: "Identification of a polymorphic gene, BCL2A1, encoding two novel hematopoietic lineage-specific minor histocompatibility antigens." in: The Journal of experimental medicine, Vol. 197, Issue 11, pp. 1489-500, 2003 (PubMed).