GABA(A) Receptor-Associated Protein-Like 2 (GABARAPL2) antibody

Antigen: GABA(A) Receptor-Associated Protein-Like 2 (GABARAPL2)

Synonyms: ATG8, GEF2, GATE16, GATE-16, Gef2, MGC108686, AI173605, MGC102013, 0610012F20Rik, 2900019O08Rik, MGC92319, zgc:92319, GABARAPL2, atg8, gef2, gate16, gate-16, MGC131197

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650659

Additional Information

Alternative name: GABARAPL2

Gene ID: 11345

UniProt: P60520

Immunogen: This GABARAPL2 antibody is generated from rabbits immunized with human GABARAPL2 recombinant protein.

Isotype: Ig

Description: Other names: GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, MAP1 light chain 3-related protein

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GABARAPL2 antibody is generated from rabbits immunized with human GABARAPL2 recombinant protein.

Molecular Weight: 13536 DA

Comments:

Background: Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of GABARAPL2 Antibody (ABIN650659) in MCF-7 cell line lysates (35ug/lane). GABARAPL2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded mouse brain tissue reacted with GABARAPL2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

GABARAPL2 Antibody (ABIN650659) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Wiemann, Arlt, Huber et al.: "From ORFeome to biology: a functional genomics pipeline." in: Genome research, Vol. 14, Issue 10B, pp. 2136-44, 2004 (PubMed).

Sou, Tanida, Komatsu et al.: "Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16." in: The Journal of biological chemistry, Vol. 281, Issue 6, pp. 3017-24, 2006 (PubMed).