|+1 404 474 4654|
|+1 888 205 9894 (TF)|
ABL2 (Arg432) antibody
Alternatives Western Blotting (WB), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This ABL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ABL2.|
|Description||Other names: Tyrosine-protein kinase ABL2, Abelson murine leukemia viral oncogene homolog 2, Tyrosine kinase ARG, ABL2, ABLL, ARG,|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This ABL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ABL2.|
|Molecular Weight||128343 DA|
Background: ABL2 is a cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of the proteins includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. ABL2 is expressed in both normal and tumor cells. The ABL2 gene product is expressed as two variants bearing different amino termini, both approximately 12-kb in length. The peptide used to generate this antibody is 100% conserved between ABL2 and ABL1.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western bloting~~1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified. The antibody is eluted with high and low pH buffers|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure|
|Restrictions||For Research Use only|
|ABL2 non P-specific Pab (ABIN650789) western blot analysis in Y79 cell line lysates (35ug/lane). ABL2(arrow) was detected using the purified Pab.|
Kruh, Perego, Miki et al.: "The complete coding sequence of arg defines the Abelson subfamily of cytoplasmic tyrosine kinases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 15, pp. 5802-6, 1990 (PubMed).
Kruh, King, Kraus et al.: "A novel human gene closely related to the abl proto-oncogene." in: Science (New York, N.Y.), Vol. 234, Issue 4783, pp. 1545-8, 1987 (PubMed).
Cao, Leng, Kufe: "Catalase activity is regulated by c-Abl and Arg in the oxidative stress response." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29667-75, 2003 (PubMed).
Cao, Leng, Liu et al.: "Catalase is regulated by ubiquitination and proteosomal degradation. Role of the c-Abl and Arg tyrosine kinases." in: Biochemistry, Vol. 42, Issue 35, pp. 10348-53, 2003 (PubMed).
|Hosts||Rabbit (26), Mouse (14)|
|Reactivities||Human (38), Mouse (Murine) (23), Rat (Rattus) (20), Pig (Porcine) (19), Dog (Canine) (18), Horse (Equine) (18), Cow (Bovine) (1), Rabbit (1)|
|Applications||Immunofluorescence (IF) (26), Western Blotting (WB) (25), ELISA (23), Immunohistochemistry (IHC) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunoprecipitation (IP) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||AA 112-259 (2), Center (2), Tyr251 (1), phosphospecific (1)|