Endophilin (pTyr80) antibody

Antigen: Endophilin

Binding Site: pTyr80

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650823

Additional Information

Gene ID: 51100

UniProt: Q9Y371

Immunogen: This Phospho-Endophilin-pY80 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 65~95 amino acids surrounding pY80 of human Endophilin.

Isotype: Ig

Description: Other names: Bax-interacting factor 1, Bif-1, SH3 domain-containing GRB2-like protein B1, Endophilin-B1, CGI-61, KIAA0491, SH3GLB1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-Endophilin-pY80 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 65~95 amino acids surrounding pY80 of human Endophilin.

Molecular Weight: 40796 DA

Comments:

Background: Endophilin may be required for normal outer mitochondrial membrane dynamics. It is required for coatomer-mediated retrograde transport in certain cells. It may recruit other proteins to membranes with high curvature and may promote membrane fusion.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-Endophilin-pY80 Pab (ABIN650823) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Takahashi, Coppola, Matsushita et al.: "Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis." in: Nature cell biology, Vol. 9, Issue 10, pp. 1142-51, 2007 (PubMed).