tyrosine Kinase, Non-Receptor, 2 (TNK2) (pTyr826) antibody

Antigen: tyrosine Kinase, Non-Receptor, 2 (TNK2)

Binding Site: pTyr826

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650839

Additional Information

Alternative name: ACK1

Gene ID: 10188

UniProt: Q07912

Immunogen: This Phospho-ACK1-Y826 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 814~844 amino acids surrounding Y826 of human ACK1.

Isotype: Ig

Description: Other names: Tyrosine kinase non-receptor protein 2, Activated CDC42 kinase 1, ACK-1, ACK1, TNK2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-ACK1-Y826 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 814~844 amino acids surrounding Y826 of human ACK1.

Molecular Weight: 114327 DA

Comments:

Background: ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-ACK1-pY826 Phospho-specific Pab (ABIN650839) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Goss, Lee, Moritz et al.: "A common phosphotyrosine signature for the Bcr-Abl kinase." in: Blood, Vol. 107, Issue 12, pp. 4888-97, 2006 (PubMed).

Wolf-Yadlin, Hautaniemi, Lauffenburger et al.: "Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 14, pp. 5860-5, 2007 (PubMed).

Huang, Mukasa, Bonavia et al.: "Quantitative analysis of EGFRvIII cellular signaling networks reveals a combinatorial therapeutic strategy for glioblastoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 31, pp. 12867-72, 2007 (PubMed).