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tyrosine Kinase, Non-Receptor, 2 (TNK2) (pTyr826) antibody
Alternatives Dot Blot (Dot), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This Phospho-ACK1-Y826 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 814~844 amino acids surrounding Y826 of human ACK1.|
|Description||Other names: Tyrosine kinase non-receptor protein 2, Activated CDC42 kinase 1, ACK-1, ACK1, TNK2|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This Phospho-ACK1-Y826 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 814~844 amino acids surrounding Y826 of human ACK1.|
|Molecular Weight||114327 DA|
Background: ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
|Dot blot analysis of anti-Phospho-ACK1-pY826 Phospho-specific Pab (ABIN650839) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.|
Goss, Lee, Moritz et al.: "A common phosphotyrosine signature for the Bcr-Abl kinase." in: Blood, Vol. 107, Issue 12, pp. 4888-97, 2006 (PubMed).
Wolf-Yadlin, Hautaniemi, Lauffenburger et al.: "Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 14, pp. 5860-5, 2007 (PubMed).
Huang, Mukasa, Bonavia et al.: "Quantitative analysis of EGFRvIII cellular signaling networks reveals a combinatorial therapeutic strategy for glioblastoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 31, pp. 12867-72, 2007 (PubMed).