Lin-28 Homolog A (C. Elegans) (LIN28A) (pSer134) antibody

Antigen: Lin-28 Homolog A (C. Elegans) (LIN28A)

Synonyms: CSDD1, LIN28, LIN-28, ZCCHC1, FLJ12457, Lin28, Tex17, Lin-28, Gm10299, AL024421, ENSMUSG00000070700, csdd1, lin-28, lin28a, zcchc1, lin28, MGC55584, sb:cb650, zgc:55584, RGD1566408, MGC97527

Binding Site: pSer134

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650868

Additional Information

Alternative name: LIN28

Gene ID: 79727

UniProt: NP_078950, Q9H9Z2

Immunogen: This Phospho-LIN28-S134 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected surrounding S134 of human LIN28.

Isotype: Ig

Description: Other names: Zinc finger CCHC domain-containing protein 1, Protein lin-28 homolog A, Lin-28A,ZCCHC1, LIN28A, CSDD1, LIN28

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-LIN28-S134 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected surrounding S134 of human LIN28.

Molecular Weight: 22743  DA

Comments:

Background: LIN28 is probable transcription factor. It plays a critical role in the control of immune response.

Application Details

Application Notes: ELISA ~~ 1:1,000 Dot blot~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-LIN28-S134 Phospho-specific Pab (ABIN650868) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Heo, Joo, Kim et al.: "TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation." in: Cell, Vol. 138, Issue 4, pp. 696-708, 2009 (PubMed).

Iliopoulos, Hirsch, Struhl: "An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation." in: Cell, Vol. 139, Issue 4, pp. 693-706, 2009 (PubMed).

Qiu, Ma, Wang et al.: "Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells." in: Nucleic acids research, Vol. 38, Issue 4, pp. 1240-8, 2010 (PubMed).