APG8b (MAP1LC3B) (pThr29) antibody

Details for Product No. ABIN650881
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Antigen
Epitope
pThr29
(1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(4), (1)
Host
Rabbit
(4)
Clonality (Clone)
Polyclonal ()
Application
Dot Blot (DB)
(2), (2), (2), (2)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN650881
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Immunogen This APG8b(MAP1LC3B) Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T29 of human APG8b(MAP1LC3B).
Clone RB28013
Isotype Ig
Specificity This Phospho-APG8b(MAP1LC3B)-T29 Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected surrounding T29 of human APG8b.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Synonyms: Microtubule-associated proteins 1A/1B light chain 3B,MAP1LC3B,MAP1ALC3
Molecular Weight 14688 DA
Gene ID 81631
NCBI Accession NP_073729
UniProt Q9GZQ8
Research Area Cell Structure, Autophagy
Application Notes DB = 1:500
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-APG8b (MAP1LC3B) (pThr29) antibody Dot blot analysis of anti-Phospho-APG8b(MAP1LC3B)-T29 Antibody Phospho-specific Pab (ABIN650881) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6 µg/mL.
Background publications Kirkin, Lamark, Sou et al.: "A role for NBR1 in autophagosomal degradation of ubiquitinated substrates." in: Molecular cell, Vol. 33, Issue 4, pp. 505-16, 2009 (PubMed).

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