WD Repeat Domain Containing 82 (WDR82) (N-Term) antibody

Antigen: WD Repeat Domain Containing 82 (WDR82)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651007

Additional Information

Alternative name: WDR82

Gene ID: 80335

UniProt: Q6UXN9

Immunogen: This WDR82 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~31 amino acids from the N-terminal region of human WDR82.

Isotype: Ig

Description: Other names: Swd2, WD repeat-containing protein 82,UNQ9342/PRO34047, WDR82A, WDR82

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This WDR82 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~31 amino acids from the N-terminal region of human WDR82.

Molecular Weight: 35079 DA

Comments:

Background: WDR82 is a component of the mammalian SET1A (MIM 611052)/SET1B (MIM 611055) histone H3-Lys4 methyltransferase complexes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of WDR82 Antibody (N-term) (ABIN651007) in HepG2 cell line lysates (35ug/lane). WDR82 (arrow) was detected using the purified Pab.

WDR82 Antibody (N-term) (ABIN651007) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR82 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

WDR82 Antibody (N-term) (ABIN651007) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Higa, Wu, Ye et al.: "CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation." in: Nature cell biology, Vol. 8, Issue 11, pp. 1277-83, 2006 (PubMed).

Lee, Skalnik: "Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes." in: Molecular and cellular biology, Vol. 28, Issue 2, pp. 609-18, 2008 (PubMed).

Wu, Wang, Lee et al.: "Molecular regulation of H3K4 trimethylation by Wdr82, a component of human Set1/COMPASS." in: Molecular and cellular biology, Vol. 28, Issue 24, pp. 7337-44, 2008 (PubMed).