Chromosome 19 Open Reading Frame 21 (C19orf21) (N-Term) antibody

Antigen: Chromosome 19 Open Reading Frame 21 (C19orf21)

Synonyms: DKFZp686H18209

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651044

Additional Information

Alternative name: C19orf21

Gene ID: 126353

UniProt: Q8IVT2

Immunogen: This C19orf21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70~99 amino acids from the N-terminal region of human C19orf21.

Isotype: Ig

Description: Other names: Uncharacterized protein C19orf21,C19orf21

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This C19orf21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70~99 amino acids from the N-terminal region of human C19orf21.

Molecular Weight: 75357 DA

Comments:

Background: The exact function of C19orf21 remains unknown.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of C19orf21 Antibody (N-term) (ABIN651044) in HepG2 cell line lysates (35ug/lane). C19orf21 (arrow) was detected using the purified Pab.

C19orf21 Antibody (N-term) (ABIN651044) IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C19orf21 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

C19orf21 Antibody (N-term) (ABIN651044) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).