Activating Transcription Factor 1 (AFT1) (C-Term) antibody
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| Synonyms |
TREB36, EWS-ATF1, FUS/ATF-1, Atf-1, zgc:64099, wu:fc92c07, treb36, MGC89098, ews-atf1, fus/atf-1, ATF1, MGC143110 |
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Binding Site
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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| Catalog no. |
ABIN651055 |
| Quantity |
0.1 mg (0.25 mg/ml) |
| Price |
280.50 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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ATF1
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Gene ID
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466
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UniProt
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P18846
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Immunogen
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This ATF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ATF1.
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Isotype
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Ig
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Description
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Other names: Protein TREB36, Activating transcription factor 1, Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1,ATF1
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Characteristics
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Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Specificity
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This ATF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ATF1.
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Molecular Weight
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29233 DA
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Comments
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Background: ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.
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Application Notes
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The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50
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Concentration
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0.25 mg/ml
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Purification
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Purified
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Buffer
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage
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Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
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Research Area
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Translation Factors, Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Signaling, Cell Structure
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Restrictions
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For Research Use only
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Product
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Zheng, Cho, Lau et al.: "Cyclin-dependent kinase 3-mediated activating transcription factor 1 phosphorylation enhances cell transformation." in: Cancer research, Vol. 68, Issue 18, pp. 7650-60, 2008 (PubMed).
Vega, Salas, Milne et al.: "Evaluating new candidate SNPs as low penetrance risk factors in sporadic breast cancer: a two-stage Spanish case-control study." in: Gynecologic oncology, Vol. 112, Issue 1, pp. 210-4, 2008 (PubMed).
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Alternatives for antigen "Activating Transcription Factor 1 (AFT1)", type "Antibodies"
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Hosts
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Rabbit (47), Goat (3), Mouse (3), Sheep (1)
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Reactivities
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Human (52), Mouse (Murine) (34), Rat (Rattus) (29), Cow (Bovine) (3), Xenopus laevis (3), Zebrafish (2), Dog (Canine) (1), Pig (Porcine) (1), Yeast (1)
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Applications
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Western Blotting (WB) (38), ELISA (25), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (IHC) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (2), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (21), pSer63,Internal Region (3), Internal Region (2), pSer63 (2), N-Term (1)
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Alternatives 
