YMEL1 (N-Term) antibody

Antigen: YMEL1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Hamster

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN651076

Additional Information

Gene ID: 10730

UniProt: Q96TA2

Immunogen: This YMEL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 140-170 from the N-terminal region of human YMEL1.

Isotype: Ig

Description: Other names: Presenilin-associated metalloprotease, PAMP, Meg-4, ATP-dependent metalloprotease FtsH1, YME1-like protein 1, ATP-dependent metalloprotease YME1L1,UNQ1868/PRO4304, YME1L, FTSH1, YME1L1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This YMEL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 140-170 from the N-terminal region of human YMEL1.

Molecular Weight: 86455 DA

Comments:

Background: YMEL1 is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Chromatin, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

YMEL1 Antibody (N-term) (ABIN651076) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of YMEL1 Antibody (N-term) (ABIN651076) in HepG2 cell line lysates (35ug/lane). YMEL1 (arrow) was detected using the purified Pab.

Western blot analysis of YMEL1 Antibody (N-term) (ABIN651076) in CHO cell line lysates (35ug/lane). YMEL1 (arrow) was detected using the purified Pab.

Publications

Product: Deloukas, Earthrowl, Grafham et al.: "The DNA sequence and comparative analysis of human chromosome 10." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004 (PubMed).

Grupe, Li, Rowland et al.: "A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease." in: American journal of human genetics, Vol. 78, Issue 1, pp. 78-88, 2005 (PubMed).