N-Acetyltransferase 8 (NAT8) (Center) antibody

Antigen: N-Acetyltransferase 8 (NAT8)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN651148

Additional Information

Alternative name: NAT8

Gene ID: 9027

UniProt: Q9UHE5

Immunogen: This NAT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 120-150 from the Central region of human NAT8.

Isotype: Ig

Description: Other names: Camello-like protein 1, Probable N-acetyltransferase 8,TSC501, GLA, CML1, NAT8

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NAT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 120-150 from the Central region of human NAT8.

Molecular Weight: 25619 DA

Comments:

Background: This protein, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Cell Structure, Protein Modifications, Cell Cycle

Restrictions: For Research Use only

Images

Western blot analysis of NAT8 Antibody (Center) (ABIN651148) in ZR-75-1 cell line lysates (35ug/lane). NAT8 (arrow) was detected using the purified Pab.

NAT8 Antibody (Center) (ABIN651148) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NAT8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Barrios-Rodiles, Brown, Ozdamar et al.: "High-throughput mapping of a dynamic signaling network in mammalian cells." in: Science (New York, N.Y.), Vol. 307, Issue 5715, pp. 1621-5, 2005 (PubMed).

Juhanson, Kepp, Org et al.: "N-acetyltransferase 8, a positional candidate for blood pressure and renal regulation: resequencing, association and in silico study." in: BMC medical genetics, Vol. 9, pp. 25, 2008 (PubMed).

Ko, Puglielli: "Two endoplasmic reticulum (ER)/ER Golgi intermediate compartment-based lysine acetyltransferases post-translationally regulate BACE1 levels." in: The Journal of biological chemistry, Vol. 284, Issue 4, pp. 2482-92, 2009 (PubMed).