ASM3A (C-term) antibody

Antigen: ASM3A (C-term)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN651168

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 10924

Swiss-Prot: NP_006705

Immunogen: This ASM3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 330-360 from the C-terminal region of human ASM3A.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Acid sphingomyelinase-like phosphodiesterase 3a, ASM-like phosphodiesterase 3a,ASML3A, SMPDL3A

Molecular Weight: 51260 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ASM3A Antibody (C-term) (ABIN651168) in MDA-MB435 cell line lysates (35ug/lane). ASM3A (arrow) was detected using the purified Pab.

ASM3A Antibody (C-term) (ABIN651168) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ASM3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ASM3A Antibody (C-term) (ABIN651168) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.