Magnesium Transporter 1 (MAGT1) (N-Term) antibody

Antigen: Magnesium Transporter 1 (MAGT1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651247

Additional Information

Alternative name: MAGT1

Gene ID: 84061

UniProt: Q9H0U3

Immunogen: This MAGT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the N-terminal region of human MAGT1.

Isotype: Ig

Description: Other names: Implantation-associated protein, IAP, Magnesium transporter protein 1, MagT1,UNQ628/PRO1244, PSEC0084, IAG2, MAGT1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAGT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the N-terminal region of human MAGT1.

Molecular Weight: 38037 DA

Comments:

Background: MAGT1 encodes a magnesium cation transporter protein that localizes to the cell membrane. This protein also associates with N-oligosaccharyl transferase and therefore may have a role in N-glycosylation. Mutations in this gene cause mental retardation X-linked type 95 (MRX95). This gene may have multiple in-frame translation initiation sites, one of which would encode a shorter protein with an N-terminus containing a signal peptide at amino acids 1-29.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of MAGT1 Antibody (N-term) (ABIN651247) in mouse NIH-3T3 cell line lysates (35ug/lane).MAGT1 (arrow) was detected using the purified Pab.

MAGT1 Antibody (N-term) (ABIN651247) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Shibatani, David, McCormack et al.: "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits." in: Biochemistry, Vol. 44, Issue 16, pp. 5982-92, 2005 (PubMed).

Molinari, Foulquier, Tarpey et al.: "Oligosaccharyltransferase-subunit mutations in nonsyndromic mental retardation." in: American journal of human genetics, Vol. 82, Issue 5, pp. 1150-7, 2008 (PubMed).

Zhou, Clapham: "Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 37, pp. 15750-5, 2009 (PubMed).