PAP Associated Domain Containing 4 (PAPD4) (N-Term) antibody

Antigen: PAP Associated Domain Containing 4 (PAPD4)

Synonyms: FLJ38499

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Hamster

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651284

Additional Information

Alternative name: GLD2

Gene ID: 167153

UniProt: Q6PIY7

Immunogen: This GLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-95 amino acids from the N-terminal region of human GLD2.

Isotype: Ig

Description: Other names: Terminal uridylyltransferase 2, TUTase 2, PAP-associated domain-containing protein 4, Poly(A) RNA polymerase GLD2, hGLD-2,GLD2, PAPD4

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-95 amino acids from the N-terminal region of human GLD2.

Molecular Weight: 56028 DA

Comments:

Background: GLD2 is cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. GLD2 does not play a role in replication-dependent histone mRNA degradation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

(LEFT)Western blot analysis of GLD2 Antibody (N-term) (ABIN651284) in MCF-7 cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab. (MIDDLE)Western blot analysis of GLD2 Antibody (N-term) (ABIN651284) in mouse heart tissue lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of GLD2 Antibody (N-term) (ABIN651284) in CHO cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.

GLD2 Antibody (N-term) (ABIN651284) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Kwak, Wang, Ballantyne et al.: "Mammalian GLD-2 homologs are poly(A) polymerases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 13, pp. 4407-12, 2004 (PubMed).

Mullen, Marzluff: "Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'." in: Genes & development, Vol. 22, Issue 1, pp. 50-65, 2008 (PubMed).